Abstract Dermatophytic and keratinophilic fungi are major components of soil microflora. Keratinolytic fungi occur abundantly in the superficial soil layers of landfills and their surroundings. The aim of the present investigation is to evaluate the survival period of these pathogenic and non pathogenic dermatophytes in different culture and temperature conditions. In present research survival period of four dermatophytic and three keratinophilic fungi has been carried out in unsterilized soil, sterilized soil and Sabouraud's dextrose-agar medium at three different temperature conditions. These fungi were collected from soil samples and infected skin samples of tinea patients of Jaipur city. Bait testing (BT) and Direct soil sprinkling (DSS) methods were used for the testing of survival after every fourth month up to two years of the study. Result: Unsterilized soil and SDA medium were found more suitable for survival of all fungi. 11±1 ºC was found to be most suitable temperature condition for all tested fungi up to two years of studies. T. tonsurans has a longer survival period among all the test fungi. T. simii survived for short period of the time under different conditions.Frequently occurrence of these fungi in different localities of Jaipur district along with longer survival period in different climates concluded that Jaipur city potentially has a high risk for causing cutaneous fungal infections in human and animals.

Resumo O objetivo deste estudo foi avaliar a viabilidade das células do ligamento periodontal de dentes avulsionados armazenados em três diferentes meios. Quarenta e cinco pré-molares com formação radicular completa extraídos por razões terapêuticas foram aleatoriamente distribuídos em três grupos, de acordo com o meio de armazenagem: leite (controle), água de arroz e clara de ovo). Após armazenar os dentes avulsionados por 30 min no meio, raspas do ligamento periodontal (LPD) foram coletadas em tubos Falcon contendo 2,5 mL de solução tamponada de soro fosfatado, incubadas por 30 min e a seguir centrifugadas por 5 min a 800 rpm. A viabilidade celular foi analisada pelo método de exclusão do Azul de Trypan. A água de arroz teve um número significativamente maior de células viáveis em comparação com o leite e a clara de ovo. Não houve diferença estatisticamente significativa entre o leite e a clara de ovo. A água de arroz pode ser capaz de manter a viabilidade das células do PDL de dentes avulsionados, melhor que o leite ou a clara de ovo.

Abstract The aim of this study was to evaluate the viability of periodontal ligament cells of avulsed teeth in three different storage media. Forty-five mature premolars extracted for orthodontic therapeutic purposes were randomly and equally divided into three groups according to the storage medium: milk (control), rice water and egg white. After placing extracted teeth for 30 min in storage media, the scrapings of the periodontal ligament (PDL) were collected in Falcon tubes containing collagenase in 2.5 mL of phosphate buffer saline and were incubated for 30 min and centrifuged for 5 min at 800 rpm. Cell viability was analyzed by Trypan blue exclusion. Rice water had a significantly higher number of viable cells compared to egg white and milk. There was no statistically significant difference between egg white and milk. Rice water may be able to maintain PDL cell viability of avulsed teeth better than egg white or milk.